Glossary
Adenine
One of the nitrogenous bases of DNA. Adenine is a purine base.
Cell culture
The growth of cells in a nutrient medium.
Cleave
To cut or split
Clone
To make an identical copy of or to duplicate.
Clones
Organisms with genetically identical DNA.
Cytosine
One of the nitrogenous bases of DNA. Cytosine is a pyrimidine base.
Deoxyribonucleic acid (DNA)
The molecule that is the building block of life. DNA is composed of three subunits.
Enzymes
Proteins that speed up a chemical reaction.
Free Floating Nucleotides
Nitrogenous bases of DNA that are in the nucleus of the cell. They are not attached to anything.
Gene Therapy
The treatment of certain disorders by inserting specific engineered genes into a patients cells.
Guanine
One of the nitrogenous bases of DNA. Guanine is a purine base.
Human Genome
Approximately 100,000 genes on 46 human chromosomes, made of about 3 billion DNA base pairs.
Lagging Strand
The strand of DNA that builds 3' to 5'.
Leading strand
The strand of DNA that builds 5' to 3'.
Nitrogenous base
A carbon ring structure found in DNA that contains one or more atoms of nitrogen.
Okazaki fragments
Short fragments of DNA produced during DNA replication. They are located on the lagging strand.
Plasmid
The small ring of DNA that can serve as a vector (usually in bacteria cells).
Purine
A double-ringed, crystalline organic base
Pyrimidine
A single-ringed, crystalline organic base
Recombinant DNA
DNA produced when a cleaved DNA fragment is incorporated into the DNA of a plasmid or virus.
Restriction Enzyme
Bacterial proteins that cleave DNA at specific pints in the nucleotide sequence.
Splice
To cut a DNA fragment.
Splicing
The rejoining of cut DNA fragments.
Thymine
One of the nitrogenous bases of DNA. Thymine is a pyrimidine base.
Vector
Any substance or organism that carries recombinant DNA to the host cell.



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